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  • Autoclave Performance Verification Log For Biohazard Waste ... - Ncsu

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Autoclave Performance Verification Log for Biohazard Waste In event of failure, contact: In accordance with NC State University Biosafety Manual Autoclave Building, Room (and/or serial) # , Year:.

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Autoclaves use saturated steam under pressure of approximately 15 pounds per square inch to achieve a chamber temperature of at least 250°F (121°C) for a prescribed time—usually 30–60 minutes. In addition to proper temperature and time, prevention of entrapment of air is critical to achieving sterility.

ONLY use autoclavable biohazard bags. Use a heat resistant SECONDARY CONTAINER to retain any leakage that may occur. DO NOT overfill bags or autoclave chamber as this decreases its effectiveness. Leave bags unsealed to allow for steam penetration.

Autoclave gauges are not always accurate, and autoclave tape only indicates the presence of hot steam. Therefore, the autoclave must be validated on a regular basis with a biological indicator ampoule or chemical integrator strip that clearly demonstrates that the appropriate conditions were achieved for sterilization.

In the healthcare industry, an autoclave is a machine that uses high pressure steam for a very specific period of time and temperature to kill pathogens, such as bacteria or viruses. This process disinfects the waste prior to final disposal.

Optimally, the waste should be exposed to: 121 degrees C, at a pressure of 15 PSIG for at least 20 minutes. Autoclave gauges are not always accurate, and autoclave tape only indicates the presence of hot steam.

Autoclave waste no less than 30 minutes at 121°C and 15psi pressure. After autoclaving, Record the run in the log, carefully remove the load, and affix a "treated" sticker to biobags. Place the biobag in a black trash bag. Lab personnel can then discard waste in the dumpster.

Temperature: Sterilizing cycle temperature must be at least 250°F (121°C). Time: The sterilizing cycle must be at least 30 minutes.

Sharps, including needles and syringes, can also be processed in an autoclave if they are not contaminated with hazardous waste.

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© Copyright 1997-2025
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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232