Get Tube Method Of Blood Grouping Pdf

The tube method involves mixing a suspension of red blood cells with antisera (or plasma, for reverse grouping) in a test tube. The mixture is centrifuged to separate the cells from the reagent, and then resuspended by gently agitating the tube.

Manual tube testing An individual's red cells (in a 2%-5% saline suspension) are mixed in a test tube with anti-D reagent. The specimen is mixed gently. After centrifugation, the pelleted cell button is gently resuspended and examined for agglutination. Agglutination indicates a positive reaction.

Disadvantages are that the testing must be done at the time of obtaining the sample, whereas tube testing allows for storage and testing at a later date.

Test tube method of blood grouping is more reliable than slide method. This is because centrifugation enhances the reaction by bringing antigen and antibodies closer together and allows detection of weaker antigen antibody reactions; in addition drying is avoided and smaller amounts of reagent are required.

The tube method involves mixing a suspension of red blood cells with antisera (or plasma, for reverse grouping) in a test tube. The mixture is centrifuged to separate the cells from the reagent, and then resuspended by gently agitating the tube.

ABO and Rh testing require both serum and red blood cells; therefore, the specimen should be collected in a vacuum tube with no preservative (red topped tube). EDTA tubes (lavender topped) may also be used. If using a red-topped tube, DO NOT USE GEL SEPARATOR TUBES.

GEL testing has a number of important advantages over routine TUBE testing, particularly when testing large numbers of samples. These include standardization, stability, smaller sample volume, ease of performance and analysis, and rapidity.

In forward grouping, blood cells are placed in two test tubes along with saline as a diluent media, and then one drop of each anti-A and anti-B is added separately in these samples. These tubes are subjected to centrifugation for few minutes, and then, the resultant matrix is gently shaken for observing agglutination.