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Get Antibody Identification Principle

Ge the tubes for 15-20 seconds on high (3500rpm). The RBCs will form a button or pellet at the bottom of the tube. 6. Examine the supernatant for hemolysis. Gently resuspend each RBC button and examine for macroscopic agglutination. Grade reactions and record results on the appropriate antigram (antigen profile sheet). 7. Add 2 drops of potentiator to each tube. Incubate at 370C for 15-30 minutes. NOTE: The addition of a potentiator is not mandatory. If no potentiator is used, incubate for 3.

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