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P-1 (ref. 12) sites are recognized through direct interactions with five conserved amino acid residues in the signature sequence of the bZIP basic regions, namely NXXAAXXCR (X represents variable amino acid residue type). Interestingly, in the signature sequence of Pap1 subfamily members, the second Ala is replaced with Gln and the Cys is replaced with Phe to give the sequence NXXAQXXFR. The characteristic DNA recognition by members of the Pap1 subfamily seems, therefore, to be an intrinsic prop.

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