Get Manual Of Mirdeepfinder For Est Or Gss

This guide provides a comprehensive overview of the Manual Of MiRDeepFinder For EST Or GSS, focusing on the essential components and instructions for completion. Users of various experience levels will find helpful, step-by-step guidance for navigating the process effectively.

Follow the steps to fill out the Manual Of MiRDeepFinder For EST Or GSS online.

1. Click the ‘Get Form’ button to obtain the form and open it in your preferred online editor.
2. Review the document’s description to understand the miRDeepFinder's purpose and installation requirement. Make sure to identify whether you are using a Windows or Linux system.
3. Prepare the necessary software and dependencies, as specified in Section 2. Identify any Perl modules and databases that need to be installed on your system.
4. Organize your data files by creating a directory structure for short read sequences, cDNA, databases, and configuration files as recommended in the workflow section.
5. Create and customize the configuration file based on your environment settings and file locations as outlined in Section 4.1.10.
6. Load the required databases into MySQL by following the instructions in Sections 4.1.11 and 4.1.12, ensuring that the paths to your files are correct.
7. Execute the initial miRNA analysis script as described in Section 5.1, ensuring that you use the correct command in the terminal.
8. Review the generated PDF reports of secondary hairpin structures to check their accuracy according to the criteria stated in Section 5.2.
9. Filter out any miRNAs with imperfect hairpin structures using the provided command in Section 5.3.
10. Group and rename your miRNAs based on the output files and instructions given in Sections 5.4 and 5.5.
11. Run the target analysis scripts as outlined in Section 5.6 and proceed with the subsequent steps to analyze GO and KEGG pathways as in Sections 5.7 and 5.8.
12. Finally, save, download, print, or share your completed results and findings as indicated in Section 5.9.

Complete the necessary documents online to facilitate your miRNA analysis using miRDeepFinder.