Get Real-time Pcr Submission Form - 384 Well Plate - Cancer Osu

Filling out the Real-Time PCR Submission Form - 384 Well Plate - Cancer Osu is a crucial step for users preparing for PCR runs. This guide provides detailed instructions on how to complete each section of the form accurately and efficiently.

Follow the steps to accurately complete the online form.

1. Use the ‘Get Form’ button to access the REAL-TIME PCR submission form and open it in your document editor.
2. Begin by entering your personal information in the designated fields, including your name, PI (principal investigator), date, phone number, time, and machine number.
3. Next, fill in the billing information. Provide details about your department, whether you are a CCC member, organization, fund, project, account number, budget year, and program.
4. Indicate your choices for plate information, selecting from the available options of Taqman, SYBR Green, or Other. Specify whether you require relative or absolute quantification along with any allelic discrimination needs.
5. Detail the reporter/quencher dyes in the designated fields. Ensure you indicate on the plate map below which wells will contain each dye (A1 to P24).
6. Input the thermal cycling parameters, including sample volume and whether a dissociation curve is needed. Specify the number of cycles required.
7. In the thermal cycling table, provide the required temperatures and times for each step, including UNG incubation, AmpliTaq Gold activation, denature, anneal, and extend.
8. Review and confirm all entries are complete and correct. Remember key points such as arriving at least 5 minutes before your run and using only flat caps or optical films.
9. Decide if you would like your plate saved after the run is complete by selecting YES or NO.
10. Finally, save the changes to your form. You can download, print, or share the form as needed.

Complete your REAL-TIME PCR submission form online to ensure a smooth processing of your PCR runs.