Get Extraction Of From Atropa Belladonna

Figure 1 shows the separation of in the extract of Atropa belladonna using gradient analysis on a reversed phase column and UV detection. Conditions Absorbance mAU standard Time min Figure 1 Column 4. 6 x 75 mm Zorbax Eclipse XDB-C18 Mobile phase A 0. 05M KH2P04 in water pH 3 B acetonitrile Flow rate 1. 0 ml/min Gradient at 0 min 10 B Column wash at 23 min 60 B UV detector variable wavelength detector 210 nm standard cell Column compartment temperature 40 C Stop time 25 min Post time 5 min Injection volume 5 l Agilent Technologies Innovating the HP Way Extraction Equipment 1 g of the dried and powdered plant from Caesar Loretz GmbH Germany was refluxed for 30 min in 0. Analysis of in Belladonna Extract Atropa Belladonna by HPLC Udo Huber Pharmaceutical The tropane alkaloid is the racemic form of which occurs in Atropa belladonna Datura stramonium Hyoscyamus niger and other extracts. The plant leaves contain about 0. 3 - 0. 7 alkaloids. The very toxic compound was already used in the Middle Ages as a extract causes the pupil to enlarge. Figure 1 shows the separation of in the extract of Atropa belladonna using gradient analysis on a reversed phase column and UV detection* Conditions Absorbance mAU standard Time min Figure 1 Column 4. 6 x 75 mm Zorbax Eclipse XDB-C18 Mobile phase A 0. 05M KH2P04 in water pH 3 B acetonitrile Flow rate 1. 0 ml/min Gradient at 0 min 10 B Column wash at 23 min 60 B UV detector variable wavelength detector 210 nm standard cell Column compartment temperature 40 C Stop time 25 min Post time 5 min Injection volume 5 l Agilent Technologies Innovating the HP Way Extraction Equipment 1 g of the dried and powdered plant from Caesar Loretz GmbH Germany was refluxed for 30 min in 0. 5 M acetic acid* After cooling the pH was adjusted to 9 and the solution was extracted five times with 50 ml chloroform* After drying over sodium sulfate the solvent was removed i. vac* and the residue dissolved in 2. 5 ml methanol* After filtration 5 l of the extract were applied to HPLC. Agilent 1100 Series Quaternary pump includes vacuum degasser Thermostatted autosampler compartment detector standard flow cell 10-mm path length 13- l cell volume Alternative Binary pump Diode array detector standard flow cell Agilent ChemStation 3D software Zorbax Eclipse XDB C18 3. 5 m 4. 6 x 75 mm Agilent part number 966967-902 Recommended Guard cartridges Zorbax Eclipse XDB C18 5 m 4 x 4 mm 7995118-504 10/pk Norm Match factor 999 Sample Offset Wavelength nm 10 12. 5 15 17. 5 20 22. 5 Comparison of sample and standard spectra of The method described here shows an easy but reliable and precise Note Since the method was specifically developed on the Agilent 1100 Series system you might not be able to reproduce this analysis on an older system or even on a new system with lower performance. Analysis of in Belladonna Extract Atropa Belladonna by HPLC Udo Huber Pharmaceutical The tropane alkaloid is the racemic form of which occurs in Atropa belladonna Datura stramonium Hyoscyamus niger and other extracts. The plant leaves contain about 0. 3 - 0. 7 alkaloids. The very toxic compound was already used in the Middle Ages as a extract causes the pupil to enlarge.