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Here we describe a novel and more cost-effective method for siRNA preparation and its application in inhibition of the replication of hepatitis B virus in HepG2 cells. In this paper we describe a novel cost-effective siRNA preparation method. We showed that dsRNAs can be easily prepared by fermentation in E.coli and purified by affinity chromatography. In conclusion the novel siRNA preparation method makes it possible to produce siRNAs on a large.

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5X siRNA Buffer is appropriate for resuspension of any short, double-strand, or single-strand synthetic RNA molecule, including modified and unmodified siRNAs, RNAs, microRNA Mimics, and microRNA Hairpin Inhibitors. Buffer must be diluted with RNase-free water prior to use.

In general, 1-30 nM siRNA is a good concentration range within which to optimize transfection (10 nM is a sufficient starting point).

FlexiPlate siRNAs are preannealed and dried down from a 10 µM solution in buffer. They should be resuspended in sterile RNase-free water only (you do not need siRNA buffer to resuspend the siRNAs).

The first step in production of antiviral siRNAs is in silico selection of highly conservative sequences in the targeted virus genome in order to achieve strong antiviral activity and avoid off-target effects.

Currently, there are five methods for generating siRNAs for gene silencing studies: Chemical synthesis. In vitro transcription. Digestion of long dsRNA by an RNase III family enzyme (e.g. Dicer, RNase III) Expression in cells from an siRNA expression plasmid or viral vector.

To dilute the 5x siRNA Buffer to 1x siRNA Buffer, mix four volumes of sterile RNase-free water with one volume of 5x siRNA Buffer. The composition of the 1x siRNA Buffer is 60 mM KCl, 6 mM HEPES-pH 7.5, and 0.2 mM MgCl2.

siRNA Resuspension Protocol 1. Briefly centrifuge the tube or plate to ensure that the dried siRNA is at the bottom of the tube. 2. Resuspend the siRNA at a convenient concentration using nuclease-free water provided for a final concentration of 100 μM.

After entry into the cytoplasm, siRNA is either loaded onto RISC directly or utilize a Dicer mediated process. After RISC loading, the passenger strand departs, thereby commencing the RNA interference process via target mRNA cleavage and degradation.

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© Copyright 1997-2025
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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232