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Standard Operating Procedure for Dissolved Oxygen Micro Method Winkler Titration LG501 Revision 06 January 2007 TABLE OF CONTENTS Section Number Subject Page 1. There is no holding time. INTERFERENCES There are numerous interferences to the dissolved oxygen test including oxidizing and reducing agents. For clean samples such as lake water chemical interferences are minimal and ignored. APPARATUS Incubation bottles 60-mL such as Wheaton Scientific.

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The Winkler method involves filling a sample bottle completely with water (no air is left to bias the test). The dissolved oxygen is then "fixed" using a series of reagents that form an acid compound that is titrated.

Two methods are commonly used to determine DO concentration: (1) The iodometric method which is a titration-based method and depends on oxidizing property of DO and (2) The membrane electrode procedure, which works based on the rate of diffusion of molecular oxygen across a membrane.

Winkler Method Calculation Answer: Step 1: Determine the average volume of delivered. Step 2: Determine the number of moles of sodium thiosulfate reacted. Step 3: Determine the moles of oxygen reacted. Step 4: Calculate the concentration of the dissolved oxygen.

Standardization is based on the co-proportionation reaction of iodide with iodate, thereby forming iodine. As described above, the iodine binds with excess iodide, and the complex is titrated with thiosulfate. One mole of iodate produces three moles iodine, which are consumed by six moles of thiosulfate.

In a glass flask, titrate 201 mL of the sample with sodium thiosulfate to a pale straw color. Titrate by slowly dropping titrant solution from a calibrated pipette into the flask and continually stirring or swirling the sample water. Add 2 mL of starch solution so a blue color forms.

Dissolved oxygen levels can be measured by a basic chemical analysis method (titration method), an electrochemical analysis method (diaphragm electrode method), and a photochemical analysis method (fluorescence method). The diaphragm electrode method is the most widely used method.

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© Copyright 1997-2025
airSlate Legal Forms, Inc.
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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232