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BD Biosciences Fluorochrome Reference Chart. W. D. Stain Index D/W. Resolution sensitivity (the ability to resolve a dim positive signal from background ) is a .

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FITC (Ex-Max 494 nm/Em-Max 520 nm): isothiocyanate - has a very high efficiency of energy transfer from absorbed to emitted light and is one of the most commonly used fluorochromes.

Here the most important aspects to consider when you select a fluorescent dye: The extinction coefficient at the excitation wavelength should be as high as possible. ... The quantum yield should be as high as possible. ... The fluorescent dye should be photostable. ... Photochromic behavior. ... Live/dead cell permeability.

Select fluorophores with high extinction coefficients ( ε ) One defining factor of a fluorophore's brightness is its extinction coefficient (a measurement of the probability of absorbing a photon of light); the higher the value of the extinction coefficient the brighter the fluorophore.

FITC (Ex-Max 494 nm/Em-Max 520 nm): isothiocyanate - has a very high efficiency of energy transfer from absorbed to emitted light and is one of the most commonly used fluorochromes.

Similarly, the light produced by fluorochromes has a range of wavelengths. The emission of light from , ranges from 490 nm to 630 nm, and the emission peak is approximately 520 nm.

Several criteria guide fluorochrome selection: i) the fluorescence profiles (excitation and emission), ii) relative brightness, iii) fluorescence overlap, iv) fluorochrome stability, and v) reproducible conjugation to antibodies.

Fluorescence is described in terms of excitation and emission. A fluorochrome may be excited by a laser at a defined excitation wavelength, at which time the molecule absorbs light photons. Then, as it returns to its ground state, the fluorochrome emits those photons with less energy and at a longer wavelength.

Use the brightest fluorochrome, typically PE or PE based energy transfer conjugates, for the protein that has the lowest expression, and vice versa the dimmest fluorochrome for the most highly expressed protein. Know the specific properties of the fluorochromes you are using.

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© Copyright 1997-2025
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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232