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Name Restriction Enzyme Worksheet 1 From City Lab s Case of the Missing Crown Jewels. Trustees of Boston University A natural enemy of bacteria is a virus. To defend themselves when attacked by a virus bacteria use chemical weapons that break up the DNA of the virus. The action of the chemicals on the viral DNA is shown in the diagram 1 below DNA from virus cut TACCGGGAATTCATCCGGTGAATTCTAGCGTAC ATGGCCCTTAAGTAGGCCACTTAAGATCGCATG TACCGGG AATTCATCCGGTG GTAGGCCACTTAA AATTCTAGCGTAC GATCGCATG Use the diagram above to answer the following questions 1. The chemical that cuts the DNA is called a. 2. These enzymes cut the DNA which creates different sized. 3. The restriction enzyme used above is called EcoRI. EcoRI cuts DNA everywhere the base pattern is. 4. Another restriction enzyme is called HaeIII. It cuts DNA at the following base sequence CCGG GGCC Show the DNA fragments that would result if HaeIII was used to cut the DNA fragment shown in the first diagram above. Do you think restriction enzymes could be used to cut DNA from other organisms Explain* 6. The words BOB and MADAM are called palindromes. What are palindromes 7. Explain how palindromes are used by restriction enzymes to cut double stranded DNA. To defend themselves when attacked by a virus bacteria use chemical weapons that break up the DNA of the virus. The action of the chemicals on the viral DNA is shown in the diagram 1 below DNA from virus cut TACCGGGAATTCATCCGGTGAATTCTAGCGTAC ATGGCCCTTAAGTAGGCCACTTAAGATCGCATG TACCGGG AATTCATCCGGTG GTAGGCCACTTAA AATTCTAGCGTAC GATCGCATG Use the diagram above to answer the following questions 1. The action of the chemicals on the viral DNA is shown in the diagram 1 below DNA from virus cut TACCGGGAATTCATCCGGTGAATTCTAGCGTAC ATGGCCCTTAAGTAGGCCACTTAAGATCGCATG TACCGGG AATTCATCCGGTG GTAGGCCACTTAA AATTCTAGCGTAC GATCGCATG Use the diagram above to answer the following questions 1. The chemical that cuts the DNA is called a. 2. These enzymes cut the DNA which creates different sized. The chemical that cuts the DNA is called a. 2. These enzymes cut the DNA which creates different sized. 3. The restriction enzyme used above is called EcoRI. EcoRI cuts DNA everywhere the base pattern is. 3. The restriction enzyme used above is called EcoRI. EcoRI cuts DNA everywhere the base pattern is. 4. Another restriction enzyme is called HaeIII. It cuts DNA at the following base sequence CCGG GGCC Show the DNA fragments that would result if HaeIII was used to cut the DNA fragment shown in the first diagram above. 4. Another restriction enzyme is called HaeIII. It cuts DNA at the following base sequence CCGG GGCC Show the DNA fragments that would result if HaeIII was used to cut the DNA fragment shown in the first diagram above. Do you think restriction enzymes could be used to cut DNA from other organisms Explain* 6. The words BOB and MADAM are called palindromes. Do you think restriction enzymes could be used to cut DNA from other organisms Explain* 6. The words BOB and MADAM are called palindromes. What are palindromes 7. Explain how palindromes are used by restriction enzymes to cut double stranded DNA.

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