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Get University Of Missouri Lidr Immunology Core Flow Cytometry Information Sheet 2018-2025
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How to fill out the University Of Missouri LIDR Immunology Core Flow Cytometry Information Sheet online
Filling out the University Of Missouri LIDR Immunology Core Flow Cytometry Information Sheet accurately is essential for smooth processing of your flow cytometry experiments. This guide provides clear, step-by-step instructions to assist you in completing the form online, ensuring all necessary information is captured properly.
Follow the steps to successfully complete the flow cytometry information sheet.
- Click ‘Get Form’ button to obtain the form and open it in an editing tool.
- Enter your personal information in the provided fields including user name, date, email, and phone number. This is crucial for future correspondence.
- Fill out the Principal Investigator (PI) section, providing the name of the PI overseeing the project along with the MoCode or purchase order (PO) number, and the associated department.
- In the project title/nickname field, provide a concise name or title for your project. Next, describe the experimental goal of your analysis and/or sort to clarify your objectives.
- Under biosafety information, indicate the biosafety level of the samples you will be bringing. Select BSL-1, BSL-2, or BSL-3 based on the nature of the samples. Be sure to respond to the questions about the origin of the samples and if they contain any genetic material.
- For cell information, indicate if the cells need sorting and specify the type of cells involved (bacteria, cell line, primary cells, or other) and their species. Additionally, indicate if the cells have been infected or fixed.
- Describe the cell's adhesiveness and provide the estimated cell size. Ensure to mention if the sample has undergone filtering, including the filter size if applicable.
- Provide the PI's signature and date in the respective fields to authenticate the submission.
- Complete the fluorescence parameters section, detailing the necessary controls for both single-color and multi-color experiments. Specify the dyes or fluorochromes used along with compensation controls.
- Address the viability dye question if applicable, stating which viability dye is being utilized. Follow this by detailing your experimental controls and samples.
- For operator use only, be prepared to fill in the appointment time and any issues encountered with the instrument along with the relevant timings.
- After completing all fields, ensure that you save your changes and consider downloading or printing a copy for your records before submission.
Complete your forms online today for efficient document handling!
Flow cytometry is a powerful technique that is widely used to identify and characterize different immune cell types in heterogeneous samples. It primarily relies on the use of fluorochrome-conjugated antibodies to detect the expression of specific cell surface or intracellular antigens on single cells in suspension.
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