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The preeminent goal in preparing samples for imaging with a confocal microscope is to maximize the fluorescence signals while pre- serving the three-dimensional structure of the specimen. Ideally, the sample should be less than ∼50 μm in thickness, although thicker samples can be visualized.

How much does a confocal microscope cost? A confocal microscope can cost anywhere from $10,000 to $100,000.

The basic components of a modern confocal microscope are the pinholes, the objective lenses, and low-noise detectors in common with the original design but also typically include fast scanning mirrors, filters for wavelength selection, and laser illumination.

Coupling of aperture-limited point scanning to a pinhole spatial filter at the conjugate image plane is an essential feature of the confocal microscope.

A confocal microscope uses a laser light source, pinhole spatial filters, multiple objective lenses, and fluorescent dyes to generate a microscopic image. The laser provides excitation light, which reflects off of a mirror. The laser light then hits two mirrors that are mounted.

For reference purposes, the confocal system should have a digital file of an image of the test specimen accessible to users of the microscope, including all parameters of its collection, such as laser power, pinhole diameter, objective lens and zoom value, and gain and black level of the detector.

CPT® Code 96932 in section: Reflectance confocal microscopy (RCM) for cellular and sub-cellular imaging of skin.

Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation.

The computer has a map, or “point spread function” of how light passes through a particular microscope. The software uses this map to determine what light is out of focus in origin and removes it through various algorithms.

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© Copyright 1997-2025
airSlate Legal Forms, Inc.
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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232