Get Spore Test Log Sheet

In-office spore testing procedure Place the test ampoule in the autoclave per the IFU. Run the autoclave using the correct cycle and parameters. Remove the ampoule from the autoclave. Crush the test and control ampoules to release the enzyme that causes an enzymatic reaction and therefore fluorescence.

In-office spore testing procedure Place the test ampoule in the autoclave per the IFU. Run the autoclave using the correct cycle and parameters. Remove the ampoule from the autoclave. Crush the test and control ampoules to release the enzyme that causes an enzymatic reaction and therefore fluorescence.

If the repeat spore test result is positive, do not use the sterilizer until it has been inspected or repaired and rechallenged with spore tests in three consecutive fully loaded chamber sterilization cycles.

If the repeat spore test result is positive, do not use the sterilizer until it has been inspected or repaired and rechallenged with spore tests in three consecutive fully loaded chamber sterilization cycles.

A negative test result (no spore growth) means that the sterilizer is operating properly. Positive test results (spore growth observed) mean that the sterilizer has failed and is not operating properly.

A negative test result (no spore growth) means that the sterilizer is operating properly. Positive test results (spore growth observed) mean that the sterilizer has failed and is not operating properly.

Biological Indicators Biological indicator vials contain spores from B. stearothermophilus, a microorganism that is inactivated when exposed to 121.1oC saturated steam for a minimum of 20 minutes. Autoclaves used to treat biological waste will be evaluated with a biological indicator by EHS on a quarterly basis.

Biological Indicators Biological indicator vials contain spores from B. stearothermophilus, a microorganism that is inactivated when exposed to 121.1oC saturated steam for a minimum of 20 minutes. Autoclaves used to treat biological waste will be evaluated with a biological indicator by EHS on a quarterly basis.

Biological indicators, or spore tests, are the most accepted means of monitoring sterilization because they assess the sterilization process directly by killing known highly resistant microorganisms (e.g., Geobacillus or Bacillus species).

Biological indicators, or spore tests, are the most accepted means of monitoring sterilization because they assess the sterilization process directly by killing known highly resistant microorganisms (e.g., Geobacillus or Bacillus species).

Spore testing assesses the killing of highly resistant Geobacillus and Bacillus spores. Inactivation of these microorganisms strongly implies that other potential pathogens in the load have been eliminated.

Spore testing assesses the killing of highly resistant Geobacillus and Bacillus spores. Inactivation of these microorganisms strongly implies that other potential pathogens in the load have been eliminated.

The Verify Spore Test Strip contains a known number of bacterial spores (at least 5 log10 or 105 per strip) of known resistance (Geobacillus stearothermophilus) to a liquid chemical sterilant used in a defined processing system.

The Verify Spore Test Strip contains a known number of bacterial spores (at least 5 log10 or 105 per strip) of known resistance (Geobacillus stearothermophilus) to a liquid chemical sterilant used in a defined processing system.

Spore test at least once a week In addition, the Centers for Disease Control and Prevention and the American Dental Association both recommend sterilizers be monitored at least weekly with biological indicators.

Spore test at least once a week In addition, the Centers for Disease Control and Prevention and the American Dental Association both recommend sterilizers be monitored at least weekly with biological indicators.

The Control (unprocessed) vial should indicate spore growth by the media changing from purple to yellow (positive result). If the control vial does not change color, re-run the test.

The Control (unprocessed) vial should indicate spore growth by the media changing from purple to yellow (positive result). If the control vial does not change color, re-run the test.

The Control (unprocessed) vial should indicate spore growth by the media changing from purple to yellow (positive result). If the control vial does not change color, re-run the test.

The Control (unprocessed) vial should indicate spore growth by the media changing from purple to yellow (positive result). If the control vial does not change color, re-run the test.

Some spore vials can also be placed into a special incubator/reader, which delivers rapid results (in one hour). The readers displays a “red light” for positive results or a “green light” for negative results. Glass ampules are available with G. stearothermophilus spores for use with steam processes.

Some spore vials can also be placed into a special incubator/reader, which delivers rapid results (in one hour). The readers displays a “red light” for positive results or a “green light” for negative results. Glass ampules are available with G. stearothermophilus spores for use with steam processes.

The Control (unprocessed) vial should indicate spore growth by the media changing from purple to yellow (positive result). If the control vial does not change color, re-run the test.

The Control (unprocessed) vial should indicate spore growth by the media changing from purple to yellow (positive result). If the control vial does not change color, re-run the test.

In-office spore testing procedure Place the test ampoule in the autoclave per the IFU. Run the autoclave using the correct cycle and parameters. Remove the ampoule from the autoclave. Crush the test and control ampoules to release the enzyme that causes an enzymatic reaction and therefore fluorescence.

If the repeat spore test result is positive, do not use the sterilizer until it has been inspected or repaired and rechallenged with spore tests in three consecutive fully loaded chamber sterilization cycles.

A negative test result (no spore growth) means that the sterilizer is operating properly. Positive test results (spore growth observed) mean that the sterilizer has failed and is not operating properly.

Biological Indicators Biological indicator vials contain spores from B. stearothermophilus, a microorganism that is inactivated when exposed to 121.1oC saturated steam for a minimum of 20 minutes. Autoclaves used to treat biological waste will be evaluated with a biological indicator by EHS on a quarterly basis.

Biological indicators, or spore tests, are the most accepted means of monitoring sterilization because they assess the sterilization process directly by killing known highly resistant microorganisms (e.g., Geobacillus or Bacillus species).

Spore testing assesses the killing of highly resistant Geobacillus and Bacillus spores. Inactivation of these microorganisms strongly implies that other potential pathogens in the load have been eliminated.

The Verify Spore Test Strip contains a known number of bacterial spores (at least 5 log10 or 105 per strip) of known resistance (Geobacillus stearothermophilus) to a liquid chemical sterilant used in a defined processing system.

Spore test at least once a week In addition, the Centers for Disease Control and Prevention and the American Dental Association both recommend sterilizers be monitored at least weekly with biological indicators.

The Control (unprocessed) vial should indicate spore growth by the media changing from purple to yellow (positive result). If the control vial does not change color, re-run the test.

The Control (unprocessed) vial should indicate spore growth by the media changing from purple to yellow (positive result). If the control vial does not change color, re-run the test.

Some spore vials can also be placed into a special incubator/reader, which delivers rapid results (in one hour). The readers displays a “red light” for positive results or a “green light” for negative results. Glass ampules are available with G. stearothermophilus spores for use with steam processes.

The Control (unprocessed) vial should indicate spore growth by the media changing from purple to yellow (positive result). If the control vial does not change color, re-run the test.