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Most normal phase columns (ie. Cyano (CN) and Amine (NH2)) are typically shipped in 100% Methanol (unless requested otherwise) as they are usually used in RP mode by most end-users even though they are tested in NP with hexane as the mobile phase.

HPLC columns should be used in well-ventilated environments to minimize concentration of solvent fumes. The HALO C18 bonded phase is nonpolar in nature. It is best utilized with mobile phases that are mixtures of methanol and water or acetonitrile and water.

ANSWER. Methanol and acetonitrile are often used with common silica-based C18 columns. In addition, ethanol, isopropanol (IPA), tetrahydrofuran (THF), and others are also used as mobile phases for reverse-phase chromatography.

It is OK to inject samples that have been prepared in acetone onto a µBondapak C18 column. Acetone will not damage this column.

Built on the renowned Hypersil silica back- bone, Thermo Scientific Hypersil BDS (Base Deactivated Silica) is an excellent reversed phase material for a wide range of applica- tions and is one of the most popular packing materials available.

A new column contains a mix- ture of methanol and water. Initially, care should be taken not to pass any mobile phase through the column that might cause a precipitate. mRP-C18 material is compatible with water and all common organic solvents, including N, N-dimethyl-formamide (DMF), and dimethylsulfoxide (DMSO).

Keep backpressures below 3500 psi (245 bar) [maximum ? 5000 psi] unless otherwise specified in Parts I-X Avoid any sudden pressure changes ? If high backpressure is observed, reverse flush the column ? (do not try this on other manufacturers' columns) Use a backpressure regulator if you are experiencing out- ? gassing ...