Polish Columns Bulletin For Free

ANSWER. Waters Magic Mix/AAA Wash is a general cleaning solution used to remove contaminants from internal HPLC and UPLC system components. The composition of the Magic Mix/AAA Wash is equal parts : MS/HPLC-grade H2O/ACN/MeOH/IPA plus 0.1 to 2% formic acid (typically).

5.0 PROCEDURE Change the mobile phase to filtered distilled water. ... Allow the water to flow through the column at the rate of 1ml / min. ... Change the mobile phase from water to HPLC grade 80% Acetonitrile. ... Set the instrument to flow rate 1 ml/ minute and wash the column for 30 minutes.

To wash the column usewater-miscible, nonaqueous solvents such as methanol, acetonitrile, or tetrahydrofuran. If you are using a buffered aqueous mobile phase, do not jump immediately to the strong solvent.

Cleaning and Regeneration An initial washing with deionised water is used to remove organic solvent and polar impurities, followed by a flush with a 0.5 M sodium chloride solution. Finally remove the salt solution with sufficient water and equilibrate the column with 80% (v/v) acetonitrile.

Regeneration Procedure for Reversed-Phase Silica Columns Disconnect the column and reconnect it to the system, with the flow through the column in the reversed direction. ... Flush out any salts/buffers with HPLC grade water. ... Flush column with 25 mL of isopropanol. Flush column with 25 mL of methylene chloride.

Typical Regeneration Procedure for Reversed Phase Columns: Disconnect column from detector and run wash solvents into a beaker. Start with your mobile phase without buffer salts (water/organic). ... Next, use 100% organic (methanol or acetonitrile). Check pressure to see if it has returned to normal; if not, then:

A much better approach to washing the column is to first replace the buffer component of the mobile phase with water. For example, replace acetonitrile?buffer with acetonitrile?water. Wash the column with 5?10 mL of this ? just enough to wash most of the buffer out.