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How to Clean an HPLC Column - Reverse Flush Method - YouTube YouTube Start of suggested clip End of suggested clip Easiest way to remove. This is to reverse the flow on your column you can do this by taking out theMoreEasiest way to remove. This is to reverse the flow on your column you can do this by taking out the column. And seeing how the flow direction is currently.

We recommend consulting a chemical disposal company with the MSDS for the packing material and any other chemicals that might also be in the column. It may be possible to then flush the column, properly dispose of its contents and recycle the hardware.

Keep it tightly capped when not in use. For prolonged storage, use a mobile phase that inhibits bacterial and mold growth. Use the column in the direction indicated on the label. An unusually high operating pressure may indicate a plugged inlet frit and may be cleared by reversing flow through the column for 10-20 mL.

5.6 Discard of HPLC / GC column 1 Discard the column if it is not meeting the criteria of Tailing factor, theoretical plates, Resolution, Fronting, Splitting/ damage etc. 5.6. 3 Discarded columns shall be kept separately and affixed to the label as ?Discarded column-Do not Use?.

How to Clean an HPLC Column - YouTube YouTube Start of suggested clip End of suggested clip The method for reverse flush would be to start with 95. Percent water. And 5 methanol for about 10MoreThe method for reverse flush would be to start with 95. Percent water. And 5 methanol for about 10 column. Volumes. Then you do a gradient of 5 to 95 methanol for five column volumes.

To backflush a column, take the column, remove it from the system, and connect the outlet to the inlet tubing from the pump. Pump water, acetonitrile or your mobile phase through the column to dislodge the particles that are blocking the frit. Do not connect your column to the detector when you are backflushing.

Regeneration Procedure for Normal-Phase Silica Column Disconnect the column and reconnect it to the system, with the flow through the column in the reversed direction.Set the flow at 1 mL/min.Flush column with 50 mL of ethyl acetate. Reconnect the column in the proper flow direction.