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Clean the column with a water/organic solvent mixture, using the same content as in the mobile phase containing an ion-pairing reagent. For example, clean the column with 10% acetonitrile in water for at least 30 minutes. Then, clean it with water/acetonitrile = 50/50 for at least 30 minutes.

Normal phase columns should be stored in Hexane. Reversed phase stationary phases should be kept in a mixture of water / organic modifier (Acetonitrile/Methanol), free from any buffers or additives that might precipitate during storage.

We recommend consulting a chemical disposal company with the MSDS for the packing material and any other chemicals that might also be in the column. It may be possible to then flush the column, properly dispose of its contents, and recycle the hardware.

Regeneration Procedure for Normal-Phase Silica Column Disconnect the column and reconnect it to the system, with the flow through the column in the reversed direction.Set the flow at 1 mL/min.Flush column with 50 mL of ethyl acetate. Reconnect the column in the proper flow direction.

Symptoms of deterioration are poor peak shape, split peaks, shoulders, loss of resolution, decreased retention times, and high back pressure. These symptoms indicate contaminants have accumulated on the frit or column inlet, or there are voids, channels, or a depression in the packing bed.

How to Clean an HPLC Column - YouTube YouTube Start of suggested clip End of suggested clip The method for reverse flush would be to start with 95. Percent water. And 5 methanol for about 10MoreThe method for reverse flush would be to start with 95. Percent water. And 5 methanol for about 10 column. Volumes. Then you do a gradient of 5 to 95 methanol for five column volumes.

Keep it tightly capped when not in use. For prolonged storage, use a mobile phase that inhibits bacterial and mold growth. Use the column in the direction indicated on the label. An unusually high operating pressure may indicate a plugged inlet frit and may be cleared by reversing flow through the column for 10-20 mL.