Dispose Columns Charter For Free

5.6 Discard of HPLC / GC column 1 Discard the column if it is not meeting the criteria of Tailing factor, theoretical plates, Resolution, Fronting, Splitting/ damage etc. 5.6. 3 Discarded columns shall be kept separately and affixed to the label as ?Discarded column-Do not Use?.

If the column was properly flushed after use and stored in 100% organic solvent such as acetonitrile, the column will survive for many years.

Keep it tightly capped when not in use. For prolonged storage, use a mobile phase that inhibits bacterial and mold growth. Use the column in the direction indicated on the label. An unusually high operating pressure may indicate a plugged inlet frit and may be cleared by reversing flow through the column for 10-20 mL.

How to Clean an HPLC Column - YouTube YouTube Start of suggested clip End of suggested clip The method for reverse flush would be to start with 95. Percent water. And 5 methanol for about 10MoreThe method for reverse flush would be to start with 95. Percent water. And 5 methanol for about 10 column. Volumes. Then you do a gradient of 5 to 95 methanol for five column volumes.

Symptoms of deterioration are poor peak shape, split peaks, shoulders, loss of resolution, decreased retention times, and high back pressure. These symptoms indicate contaminants have accumulated on the frit or column inlet, or there are voids, channels, or a depression in the packing bed.

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