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The standard approach for measuring vector copy number (VCN) is through population analysis. In this approach, genomic DNA (gDNA) is extracted from bulk cells, and the total number of viral genomes, as determined by quantitative PCR (qPCR), represents the average of the whole population.

Measuring the number of vector copies in individual target cells is important to assess the therapeutic window of integrative vectors. Their biological potency but also their inherent risk of genotoxicity is related to the number of vector copies integrated per cell.

The standard approach for measuring vector copy number (VCN) is through population analysis. In this approach, genomic DNA (gDNA) is extracted from bulk cells, and the total number of viral genomes, as determined by quantitative PCR (qPCR), represents the average of the whole population.

The standard approach for measuring vector copy number (VCN) is through population analysis. In this approach, genomic DNA (gDNA) is extracted from bulk cells, and the total number of viral genomes, as determined by quantitative PCR (qPCR), represents the average of the whole population.

Abstract. Background: In the context of ex vivo gene therapy or chimeric antigen receptor T cell (CAR-T) cell therapy, vector copy number (VCN) analysis in transduced cells by lentiviral vectors enables the assessment of risk and therapeutic efficiency in patients.

Abstract. Background: In the context of ex vivo gene therapy or chimeric antigen receptor T cell (CAR-T) cell therapy, vector copy number (VCN) analysis in transduced cells by lentiviral vectors enables the assessment of risk and therapeutic efficiency in patients.

Vector copy number (VCN), a measurement of transgene copies within a CAR T cell product, is a product-specific characteristic that must be quantified prior to patient administration as high VCN increases the risk of insertional mutagenesis.