Adapt Columns Resolution For Free

Length of the size exclusion column will be proportional to the number of theoretical plates, so increasing length will enhance the separation resolution but upto a limit. The column width determines the limit for the sample volume that can be injected without affecting the resolution limits.

A longer column generally improves the separation. The trade-off is that the retention time increases proportionally to the column length and a significant peak broadening will be observed as well because of increased longitudinal diffusion inside the column.

Based on chromatographic theory, resolution and column length is related by a square root function.This square root relationship means that a large length of column has to be removed before a significant loss in resolution occurs. For a 30-m column, a loss of 1 m results in only a 1.7% decrease in resolution.

As the resolution is proportional to the square root of the number of theoretical plates in equation (1), doubling the number of theoretical plates increases the resolution by a factor of 221a2ffe3= 1.41; increasing the number of theoretical plates four times increases the resolution by a factor of 221a4ffe3= 2.

The resolution of a column is measured by its efficiency and the more efficient a column is the better the resolution. Column efficiency is: proportional to column length; a longer column is more efficient. inversely proportional to the stationary phase particle size.

How to Improve Resolution in HPLCIncreasing column length.Decreasing particle size.Reducing peak tailing.Increasing temperature.Reducing system extra-column volume.

To set column width with a specific measurement:Select the columns you want to modify.Click the Format command on the Home tab. The format drop-down menu appears.Select Column Width. Increasing the column width.The Column Width dialog box appears. Enter a specific measurement.Click OK.