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Uito exposure bioassays Marit Farenhorst1*, Bart GJ Knols2 Abstract Background: Interest in the use of fungal entomopathogens against malaria vectors is growing. Fungal spores infect insects via the cuticle and can be applied directly on the insect to evaluate infectivity. For flying insects such as mosquitoes, however, application of fungal suspensions on resting surfaces is more realistic and representative of field settings. For this type of exposure, it is essential to apply specific amount.
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Farenhorst FAQ
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A method of endospores staining is Schaeffer Fulton method that used Malachite Green. It is an alkaline substance staining that can staining the spore bacteria. In this research, it have found the alternative staining that can replace Malachite Green solution in spore bacterial stain.
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Fungi reproduce by spores, which are produced by either sexual or asexual methods, and the majority of fungal spores are adapted for airborne dispersal.
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THE usual method for staining bacterial and yeast spores requires three operations: the staining of both spores and vegetative cells by applying heat to the slide bearing the dye; the removal of stain from the vegetative cells with a decolorizing agent; and counterstaining the vegetative cells with a dye of a different ...
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Serial dilution is used to estimate spore concentration/ml. However, for accurate spore count, hemacytometer is generally the standard method . Hemocytometer is best for estimating the concentration of fungal spores for inoculation study or spores counting.
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Fungal Stains. Lactophenol cotton blue is a stain that is used to examine fungal elements following either a tape preparation or a scraping. This stain contains phenol, which will kill the organisms, lactic acid which preserves fungal structures, and cotton blue which stains the chitin found in the fungal cell walls.
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The staining technique of Gutstein (4) provides a convenient means for staining yeast as an aid in its microscopic examination. The solutions required are: 1% solution of methylene blue in distilled water 5% solution of tannic acid in distilled water (e.g. Mallinckrodt tannic acid N.F.
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Staining: Lactophenol cotton blue is the stain used for staining fungi. It is a mixture of methyl blue which is a histological stain and lactophenol. The staining technique of fungi using the lactophenol cotton blue is called mounting of fungus.
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A single spore is placed on plant roots with a Pasteur pipette by use of a binocular microscope, and the plant is transplanted into sand in a 50 ml growth tube. Plants are incubated for 6 to 8 weeks in a greenhouse and are continuously subirrigated by capillarity with dilute (5 to 10%) Hoagland's solution.
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Keywords relevant to A Novel Method For Standardized Application Of Fungal Spore ...
- Metarhizium
- Knols
- infectivity
- Beauveria
- substrates
- 2010
- Farenhorst
- M2
- malariajournal
- conidia
- virulence
- gambiae
- bioassays
- K-Hand
- invertebr
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