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IQUI Department of Microbiology/Immunology, University of Colorado Health Sciences Center, School of Medicine, Denver, Colorado 80262 Communicated by David Talmage, May 2, 1983 ABSTRACT DNA extracted from Kaposi sarcoma biopsies was analyzed for the presence of hepatitis B viral (HBV) DNA sequences by Southern blot hybridization. The probes used were (i) molecularly cloned subgenomic fragments of HBV DNA and (ii) HBV DNA extracted from Dane particles that included DNA sequences that encode hep.
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DHBV FAQ
Positive HBsAg indicates current infection, positive anti-HBs indicates immunity (through vaccination or past infection), and positive anti-HBc indicates past or current infection (this test may occasionally give a false-positive result).
HBV DNA is negative. To resolve the ambiguity of a false-positive total anti-HBc result, test a follow-up sample 4–8 weeks later. If found positive, interpret as a resolved infection. If negative, interpret as false-positive.
The low replication phase, being referred as the inactive HBsAg carrier state, occurs after HBeAg seroconversion and is usually characterized with a marked reduction or undetectable level of serum HBV DNA, ALT normalization and resolution of liver necroinflammation.
A negative test result indicates the virus may not be actively reproducing in the liver. In general, a person is considered very infectious when the test is positive, and less infectious when the test is negative. The loss of e-Antigen can occur naturally or as a result of drug treatment.
The presence of HBV DNA in serum is a reliable marker of active HBV replication. HBV DNA levels are detectable by 30 days following infection, generally reach a peak at the time of acute hepatitis, and gradually decrease and disappear when the infection resolves spontaneously.
The genome of HBV is a partially double-stranded circular DNA of about 3.2 kilobase (kb) pairs.
An "Undetected" result indicates that hepatitis B virus (HBV) DNA was not detected in the serum specimen. A result of "<10 IU/mL (<1.00 log IU/mL)" indicates that HBV DNA is detected, but the HBV DNA level present cannot be quantified accurately below this lower limit of quantification of this assay.
The inactive carrier state is defined by normal ALT and HBV DNA < 2000 IU/mL (10 000 copies/mL).
Siddiqui Related content
Hepatitis B virus DNA in Kaposi sarcoma
by A Siddiqui · 1983 · Cited by 105 — DNA extracted from Kaposi sarcoma biopsies was...
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