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Genetic material of ϕ×174 bacteriophage is Single stranded DNA.

The cos site represents the junction between two genomes in a concatemer and serves as the packaging initiation site. Unlike the pac sequences of viruses that use the head-full packaging mechanism, cos also serves as a specific packaging termination sequence.

A DNA sequence for the genome of bacteriophage phi X174 of approximately 5,375 nucleotides has been determined using the rapid and simple 'plus and minus' method.

Bacteriophage have either DNA or RNA as their genetic material, in either circular or linear configuration, as a single- or a double-stranded molecule.

The phi X 174 (or ΦX174) bacteriophage is a single-stranded DNA (ssDNA) virus that infects Escherichia coli, and the first DNA-based genome to be sequenced. This work was completed by Fred Sanger and his team in 1977.

Coliphage: The virus which infect Escherichia coli is called the Coliphage. It is a type of bacteriophage. The Coliphage ϕ X174 contains Single-stranded DNA made of 5386 nucleotides that encodes 11 proteins. The genome of Coliphage ϕ X174 was first sequenced by Fredrick Sanger in 1977.