Protocol Pub. No. MAN0009869 Rev. A.00 Package Contents Storage Conditions Required Materials Catalog Number 4398876 4398881 4398886 4398901 Size 40 rxns 200 rxns 2,000 rxns 2,000 rxns Kit Contents.
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How to fill out the Amplitaq Gold 360 Master Mix online
This guide provides clear and comprehensive instructions on filling out the Amplitaq Gold 360 Master Mix form online. Whether you are experienced or just starting, these steps will support you in accurately completing the form to ensure effective use of the master mix.
Follow the steps to successfully complete the online form.
- Press the ‘Get Form’ button to access the Amplitaq Gold 360 Master Mix form and open it in your preferred editing method.
- Begin by entering the catalog number corresponding to the kit size you are using: 4398876 for 40 reactions, 4398881 for 200 reactions, or 4398901 for 2,000 reactions.
- In the next section, indicate your template by selecting either cDNA, gDNA, or λDNA, based on your experiment's requirements.
- Fill in the volumes of autoclaved distilled water required to reach your final volume based on the number of reactions you are preparing.
- Complete the fields for the amount of AmpliTaq Gold 360 Master Mix and any optional 360 GC Enhancer depending on your specific targets and preferences.
- Provide information about the forward and reverse primers being used, ensuring to list the correct concentrations.
- After completing all required fields, review the information entered for accuracy. Make any necessary corrections.
- Once all steps are completed and validated, you can save the form for future reference, or proceed to download, print, or share it as needed.
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What temperature should PCR extension be?
A typical PCR cycle includes an extension step at 72°C after denaturation of double-stranded DNA and annealing of oligonucleotide primers. At this temperature the thermostable poly-merase replicates the DNA at an optimal rate that depends on the buffer and nature of the DNA template ( 1 ).
Is AmpliTaq gold a hot-start?
Applied Biosystems AmpliTaq Gold and AmpliTaq Gold 360 DNA Polymerases are hot-start enzymes which utilize a chemical modification to inhibit enzyme activity at room temperature.
What temperature should Taq polymerase be extended?
The optimal extension temperature for Taq DNA Polymerase is 70-75°C. The recommended extension step is 1 min at 72°C for PCR products up to 2 kb. For larger products, the extension time should be prolonged by 1 min/kb.
What is AmpliTaq Gold 360 Master Mix?
AmpliTaq Gold 360 Master Mix contains everything required for successful PCR amplification in one convenient package, with all components premixed and premeasured. The master mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers.
What does AmpliTaq gold do?
AmpliTaq Gold™ 360 DNA Polymerase for superior performance When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.
Why is the temperature raised to 72 degrees during the extension phase of PCR?
Extending stage During this final step, the heat is increased to 72⁰C to enable the new DNA to be made by a special Taq DNA polymerase enzyme which adds DNA bases.
What temperature should I extend my AmpliTaq gold?
The activation is performed by heating the enzyme reaction mix for 1 to 10 minutes at 95° C.
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